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Becker & Hickl flim spc 150 tcspc module
Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
Flim Spc 150 Tcspc Module, supplied by Becker & Hickl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tcspc+flim+module/tcspc+module/pmc11591579-70-16-21
Average 90 stars, based on 1 article reviews
flim spc 150 tcspc module - by Bioz Stars, 2026-09
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Images

1) Product Images from "Unraveling Microviscosity Changes Induced in Cancer Cells by Photodynamic Therapy with Targeted Genetically Encoded Photosensitizer"

Article Title: Unraveling Microviscosity Changes Induced in Cancer Cells by Photodynamic Therapy with Targeted Genetically Encoded Photosensitizer

Journal: Biomedicines

doi: 10.3390/biomedicines12112550

Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative FLIM images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
Figure Legend Snippet: Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative FLIM images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

Techniques Used: Membrane, Viscosity, Control

Plasma membrane microviscosity in HeLa tumor spheroids after PDT with KillerRed localized in the nuclei (H2B) or within the plasma membrane (PM). ( A ) Schematic representation of the spheroid area (shown by the yellow square) imaged by FLIM. The spheroid had adhered to the glass bottom, and the images were acquired from a depth of ~30 μm. Higher-magnification image of the molecular rotor distribution in spheroid cell membranes indicated by the red squares. The scale bar is 80 μm. ( B ) FLIM images and live/dead (LD) assay of control and treated cells in spheroids. Bar = 80 μm. ( C ) Morphology of control and treated spheroids. The scale bar is 80 μm. ( D ) Quantification of membrane microviscosity of spheroid cells after PDT. Means ± SD, n = 4 spheroids, 60 cells in each. ( E ) Quantitative analysis of dead cells in control and treated cell populations, %. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
Figure Legend Snippet: Plasma membrane microviscosity in HeLa tumor spheroids after PDT with KillerRed localized in the nuclei (H2B) or within the plasma membrane (PM). ( A ) Schematic representation of the spheroid area (shown by the yellow square) imaged by FLIM. The spheroid had adhered to the glass bottom, and the images were acquired from a depth of ~30 μm. Higher-magnification image of the molecular rotor distribution in spheroid cell membranes indicated by the red squares. The scale bar is 80 μm. ( B ) FLIM images and live/dead (LD) assay of control and treated cells in spheroids. Bar = 80 μm. ( C ) Morphology of control and treated spheroids. The scale bar is 80 μm. ( D ) Quantification of membrane microviscosity of spheroid cells after PDT. Means ± SD, n = 4 spheroids, 60 cells in each. ( E ) Quantitative analysis of dead cells in control and treated cell populations, %. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

Techniques Used: Membrane, Control

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Article Title: The use of DAPI fluorescence lifetime imaging for investigating chromatin condensation in human chromosomes
Article Snippet: A time-correlated single photon counting (TCSPC) FLIM module (SPCM-830) from Becker & Hickl was used to record the arrival time of the photons at each x, y scan position in a time-tagging, first-in first-out mode.

Fluorescence:

Article Title: Glioblastoma Sensitization to Therapeutic Effects by Glutamine Deprivation Depends on Cellular Phenotype and Metabolism
Article Snippet: Glutamine plays an important role in tumor metabolism.. It is known that the core region of solid tumors is deprived of glutamine, which affects tumor growth and spread.. Here we investigated the effect of glutamine deprivation on cellular metabolism and sensitivity of human glioblastoma cells U87MG and T98G to drugs of various origin: alkylating cytostatic agent temozolomide; cytokine TRAIL DR5-B – agonist of the DR5 receptor; and GMX1778 – a targeted inhibitor of the enzyme nicotinamide phosphoribosyltransferase (NAMPT), limiting NAD biosynthesis.



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Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative <t>FLIM</t> images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
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Image Search Results


Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative FLIM images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

Journal: Biomedicines

Article Title: Unraveling Microviscosity Changes Induced in Cancer Cells by Photodynamic Therapy with Targeted Genetically Encoded Photosensitizer

doi: 10.3390/biomedicines12112550

Figure Lengend Snippet: Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative FLIM images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

Article Snippet: For viscous imaging, a LSM 880 laser scanning microscope (Carl Zeiss, Gottingen, Germany) equipped with a FLIM SPC 150 TCSPC module (Becker & Hickl GmbH, Berlin, Germany) and a Mai Tai HP femtosecond laser (80 MHz, 140 fs, Spectra Physics, Milpitas, CA, USA) were used.

Techniques: Membrane, Viscosity, Control

Plasma membrane microviscosity in HeLa tumor spheroids after PDT with KillerRed localized in the nuclei (H2B) or within the plasma membrane (PM). ( A ) Schematic representation of the spheroid area (shown by the yellow square) imaged by FLIM. The spheroid had adhered to the glass bottom, and the images were acquired from a depth of ~30 μm. Higher-magnification image of the molecular rotor distribution in spheroid cell membranes indicated by the red squares. The scale bar is 80 μm. ( B ) FLIM images and live/dead (LD) assay of control and treated cells in spheroids. Bar = 80 μm. ( C ) Morphology of control and treated spheroids. The scale bar is 80 μm. ( D ) Quantification of membrane microviscosity of spheroid cells after PDT. Means ± SD, n = 4 spheroids, 60 cells in each. ( E ) Quantitative analysis of dead cells in control and treated cell populations, %. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

Journal: Biomedicines

Article Title: Unraveling Microviscosity Changes Induced in Cancer Cells by Photodynamic Therapy with Targeted Genetically Encoded Photosensitizer

doi: 10.3390/biomedicines12112550

Figure Lengend Snippet: Plasma membrane microviscosity in HeLa tumor spheroids after PDT with KillerRed localized in the nuclei (H2B) or within the plasma membrane (PM). ( A ) Schematic representation of the spheroid area (shown by the yellow square) imaged by FLIM. The spheroid had adhered to the glass bottom, and the images were acquired from a depth of ~30 μm. Higher-magnification image of the molecular rotor distribution in spheroid cell membranes indicated by the red squares. The scale bar is 80 μm. ( B ) FLIM images and live/dead (LD) assay of control and treated cells in spheroids. Bar = 80 μm. ( C ) Morphology of control and treated spheroids. The scale bar is 80 μm. ( D ) Quantification of membrane microviscosity of spheroid cells after PDT. Means ± SD, n = 4 spheroids, 60 cells in each. ( E ) Quantitative analysis of dead cells in control and treated cell populations, %. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.

Article Snippet: For viscous imaging, a LSM 880 laser scanning microscope (Carl Zeiss, Gottingen, Germany) equipped with a FLIM SPC 150 TCSPC module (Becker & Hickl GmbH, Berlin, Germany) and a Mai Tai HP femtosecond laser (80 MHz, 140 fs, Spectra Physics, Milpitas, CA, USA) were used.

Techniques: Membrane, Control